Femtosecond laser cross-linking and integrated analytical workflows for decoding the dynamic dna protein interactome

The HAWK HOCHSCHULE FUR ANGEWANDTE WISSENSCHAFT UND KUNST FACHHOCHSCHULE HILDESHEIM HOLZMINDEN GOTTINGEN in Belgium from Europe region has released this tender for Femtosecond laser cross-linking and integrated analytical workflows for decoding the dynamic dna protein interactome FEMTOMICS is redefining analytical chemistry by introducing the first femtosecond-based platform for proteome-wide decoding of dynamic DNA protein interactions in cells Capturing weak and transient interactions in intact cells remains a key challenge in measurement science Chemical cross-linking is slow perturbs cellular equilibrium and has limited specificity while UV irradiation is inefficient damaging and fails to stabilise fleeting contacts Current methods detect abundant interactions such as histones but cannot resolve many transcription factors or other DNA-binding proteins whose short-lived bindings are essential for gene regulation Moreover genomic proteomic and structural layers are typically studied in isolation overlooking integration FEMTOMICS closes these gaps with a paradigm-shifting platform that integrates femtosecond laser cross-linking fliX with high-resolution mass spectrometry fliX MS and nanopore sequencing fliX NPS With sub-ps fliX enables zero-length cross-linking of DNA protein interactions while preserving DNA integrity fliX MS provides proteome-wide detection of DNA-bound proteins at atomic resolution and fliX NPS pinpoints binding sites at single-nucleotide resolution with matched transcriptome and methylation profiles all datasets are integrated through advanced bioinformatics for structural and functional analysis These modules yield time-resolved multimodal maps of transient DNA protein complexes in situ By benchmarking against established methods FEMTOMICS will demonstrate measurable gains in temporal resolution specificity and coverage expanding detection from histones to rare transcription factors The resulting datasets will reconstruct networks generate hypotheses and resolve regulatory states As proof of concept the platform will be validated in breast cancer cell models demonstrating how photonics sequencing and data science set new standards in chemistry and medicine with tender notice no. 101267519 published on 24 Aug 2026. The tender is due to expire on 30 Nov 2031. This tender is sourced from Cordis and is eligible for suppliers interested in Ion and molecular microscopes, Proteomics, Molecular diagnostic, DNA, Sequencing. Interested bidders can obtain further information including complete bid documents or ways to participate by registering on Tender Impulse and referencing via Tender Impulse ID 14500589.